Gladis Mushroom is a member of the Mushroom Family. She is the mother of Angus and Marcus Mushroom and the wife of Lazarus Mushroom. She appears in "Larry-Boy and the Rumor Weed." She can be seen driving in a car with Angus and Marcus.She appears again at the library with her family. She believed the rumors about Alfred, but soon discovered it was a lie. She first appeared on a banner in Stuff Mart in "Madame Blueberry."
Gladis is a skinny mushroom. She is also tall and has a small nose and mouth. She is in a shade of gray, the same as her eyelids and nose. She is the skinniest in the Mushroom Family.
- Herself in "Madame Blue Berry" (banner)
- Herself in "Larry-Boy and the Rumor Weed" (cameo)